Split GFP system for direct quantification of protein expression and qualitative yield estimation
Split GFP quantification provides direct quantification of protein expression using a split green fluorescent protein system. We use this technology for expression validation and qualitative yield estimation.
The split GFP system divides the green fluorescent protein into two non-fluorescent fragments. When these fragments are brought together through protein expression, they reconstitute to form a functional, fluorescent GFP. The fluorescence intensity correlates with the amount of expressed protein.
Split GFP quantification works alongside our other technologies to provide comprehensive protein expression analysis, complementing cell-free expression systems and other analytical methods for complete protein characterization workflows.