Split GFP Quantification
Split GFP system for direct quantification of protein expression and qualitative yield estimation
Split GFP quantification provides direct quantification of protein expression using a split green fluorescent protein system. We use this technology for expression validation and qualitative yield estimation.
How It Works
The split GFP system divides the green fluorescent protein into two non-fluorescent fragments. When these fragments are brought together through protein expression, they reconstitute to form a functional, fluorescent GFP. The fluorescence intensity correlates with the amount of expressed protein.
Applications
Expression Validation
Confirm successful protein expression and validate expression systems.
Yield Estimation
Qualitative assessment of protein expression yields across different conditions.
Integration
Split GFP quantification works alongside our other technologies to provide comprehensive protein expression analysis, complementing cell-free expression systems and other analytical methods for complete protein characterization workflows.