Binding Screening
Rapid, cost-effective screening to identify and rank protein binding interactions
Binding Screening
Binding screening is our most popular service, designed for rapid identification and ranking of protein binding interactions. This cost-effective assay is ideal for initial screening of new designs, library exploration, and lead identification campaigns.
How It Works
Binding screening uses a streamlined protocol with two analyte concentrations to quickly categorize your protein variants into binding strength buckets. This approach provides essential binding information while maintaining high throughput and cost efficiency.
Protein Expression
Your protein sequences are expressed using our cell-free system and immobilized on BLI biosensor tips via C-terminal tags.
Binding Assessment
Sensors are exposed to two different concentrations of your target protein to assess binding strength and kinetics.
Rapid Analysis
Binding responses are analyzed to categorize variants as Strong, Medium, Weak, or Non-binding.
Binding Classification
Our binding screening categorizes your variants into clear, actionable groups:
KD < 50 nM
High-affinity interactions suitable for therapeutic applications or high-sensitivity assays.
Characteristics:
- Robust binding signals
- Clear association and dissociation phases
- Excellent signal-to-noise ratios
- High confidence in measurements
KD < 50 nM
High-affinity interactions suitable for therapeutic applications or high-sensitivity assays.
Characteristics:
- Robust binding signals
- Clear association and dissociation phases
- Excellent signal-to-noise ratios
- High confidence in measurements
KD 50 nM - 1 μM
Moderate-affinity interactions suitable for many applications and further optimization.
Characteristics:
- Clear binding responses
- Well-defined kinetic phases
- Good optimization potential
- Suitable for most applications
KD > 1 μM
Lower-affinity interactions that may require optimization or serve specialized purposes.
Characteristics:
- Detectable binding responses
- May show faster dissociation
- Potential for improvement
- Application-dependent utility
No Detectable Binding
Variants showing no significant binding above background levels.
Characteristics:
- Flat binding curves
- Signals at background levels
- Clear candidates for elimination
- May indicate expression issues
Key Applications
Library Screening
Screen large libraries of protein variants to identify the most promising candidates for further development.
Lead Identification
Rapidly identify binding hits from diverse design approaches or computational predictions.
Comparative Analysis
Compare binding performance across different protein scaffolds, mutations, or design strategies.
Quality Control
Validate protein expression and basic binding functionality before investing in detailed characterization.
Advantages of Binding Screening
Speed and Efficiency
- Rapid turnaround: Results in 2-3 weeks
- High throughput: Process 100+ variants efficiently
- Streamlined analysis: Clear categorization without complex fitting
Cost Effectiveness
- Lower per-variant cost compared to full characterization
- Volume discounts for larger screening campaigns
- Risk reduction by identifying non-binders early
Decision Support
- Clear ranking of variant performance
- Actionable categories for follow-up decisions
- Statistical confidence in relative rankings
When to Use Binding Screening
Initial Library Exploration
Initial Library Exploration
Perfect for first-pass screening of large variant libraries to identify promising regions for focused optimization.
Typical project size: 50-500 variants Goal: Identify top 10-20% performers for further study
Design Validation
Design Validation
Validate computational predictions or design hypotheses before investing in detailed characterization.
Typical project size: 20-100 variants Goal: Confirm predicted binding behavior
Comparative Studies
Comparative Studies
Compare binding performance across different protein formats, scaffolds, or optimization strategies.
Typical project size: 50-200 variants Goal: Identify most promising approaches
Quality Assessment
Quality Assessment
Screen protein variants to identify those suitable for downstream applications or further optimization.
Typical project size: 10-100 variants Goal: Select well-behaved, binding-competent variants
Complementary Assays
Binding screening often serves as the first step in a comprehensive characterization workflow:
- Affinity Characterization: Detailed kinetic analysis of top performers
- Epitope Binning: Map binding sites of interesting variants
- Thermostability: Assess stability of promising candidates
Data Delivery
Your binding screening results include:
- Binding classification for each variant (Strong/Medium/Weak/Non-binding)
- Relative ranking within each category
- Raw binding curves for visual inspection
- Expression level assessment for each variant
- Summary statistics and quality metrics
- Recommendations for follow-up experiments
Getting Started
Ready to screen your protein variants?
- Prepare your sequences in FASTA or CSV format
- Select your target from our library or provide your own
- Configure your experiment through the Foundry Portal
- Review and submit for rapid turnaround
Binding screening is ideal for new customers or new protein types. Start here to understand how your variants perform on our platform before investing in more detailed assays.
======= description: Protein binding screening is designed to quickly and efficiently evaluate large libraries of protein variants for their ability to bind a target of interest.
This high-throughput workflow enables you to classify candidates as binders or non-binders while also categorizing their binding strength (e.g., weak, medium, or strong). By focusing on rapid and cost-effective assays, our screening service is ideal for early-stage discovery projects where prioritizing candidates is key.
Key Features:
-
Starts at 99$/protein. 21 days turnaround time.
-
Yes/No Binding Classification: Rapidly determine whether each variant binds to the target.
-
Binding Strength Categorization: Classifies binders as weak, medium, or strong (no KD values).
-
High Throughput: Capable of screening thousands of variants in a single campaign.
-
Cost-Effective: Optimized for large-scale projects without compromising data quality.
When to Use:
-
To triage large libraries of protein variants during early discovery phases.
-
To identify promising binders for subsequent detailed characterization.
-
When rapid and broad insights into binding potential are required.
-
If you need precise binding kinetics with KD values, see Affinity Characterization.
How It Works
During a binding screen, protein variants are immobilized or presented in solution, and the target analyte is flowed over the experimental setup. Real-time binding events are monitored using bio-layer interferometry (BLI), and results are analyzed to classify each variant. Single-concentration measurements provide approximate binding strength, allowing you to prioritize strong binders while excluding non-binders or weak candidates
131ed47d083be70c654c1ff1f875b4f3206dfbf8
Binding Screening
Rapid, cost-effective screening to identify and rank protein binding interactions
Binding Screening
Binding screening is our most popular service, designed for rapid identification and ranking of protein binding interactions. This cost-effective assay is ideal for initial screening of new designs, library exploration, and lead identification campaigns.
How It Works
Binding screening uses a streamlined protocol with two analyte concentrations to quickly categorize your protein variants into binding strength buckets. This approach provides essential binding information while maintaining high throughput and cost efficiency.
Protein Expression
Your protein sequences are expressed using our cell-free system and immobilized on BLI biosensor tips via C-terminal tags.
Binding Assessment
Sensors are exposed to two different concentrations of your target protein to assess binding strength and kinetics.
Rapid Analysis
Binding responses are analyzed to categorize variants as Strong, Medium, Weak, or Non-binding.
Binding Classification
Our binding screening categorizes your variants into clear, actionable groups:
KD < 50 nM
High-affinity interactions suitable for therapeutic applications or high-sensitivity assays.
Characteristics:
- Robust binding signals
- Clear association and dissociation phases
- Excellent signal-to-noise ratios
- High confidence in measurements
KD < 50 nM
High-affinity interactions suitable for therapeutic applications or high-sensitivity assays.
Characteristics:
- Robust binding signals
- Clear association and dissociation phases
- Excellent signal-to-noise ratios
- High confidence in measurements
KD 50 nM - 1 μM
Moderate-affinity interactions suitable for many applications and further optimization.
Characteristics:
- Clear binding responses
- Well-defined kinetic phases
- Good optimization potential
- Suitable for most applications
KD > 1 μM
Lower-affinity interactions that may require optimization or serve specialized purposes.
Characteristics:
- Detectable binding responses
- May show faster dissociation
- Potential for improvement
- Application-dependent utility
No Detectable Binding
Variants showing no significant binding above background levels.
Characteristics:
- Flat binding curves
- Signals at background levels
- Clear candidates for elimination
- May indicate expression issues
Key Applications
Library Screening
Screen large libraries of protein variants to identify the most promising candidates for further development.
Lead Identification
Rapidly identify binding hits from diverse design approaches or computational predictions.
Comparative Analysis
Compare binding performance across different protein scaffolds, mutations, or design strategies.
Quality Control
Validate protein expression and basic binding functionality before investing in detailed characterization.
Advantages of Binding Screening
Speed and Efficiency
- Rapid turnaround: Results in 2-3 weeks
- High throughput: Process 100+ variants efficiently
- Streamlined analysis: Clear categorization without complex fitting
Cost Effectiveness
- Lower per-variant cost compared to full characterization
- Volume discounts for larger screening campaigns
- Risk reduction by identifying non-binders early
Decision Support
- Clear ranking of variant performance
- Actionable categories for follow-up decisions
- Statistical confidence in relative rankings
When to Use Binding Screening
Initial Library Exploration
Initial Library Exploration
Perfect for first-pass screening of large variant libraries to identify promising regions for focused optimization.
Typical project size: 50-500 variants Goal: Identify top 10-20% performers for further study
Design Validation
Design Validation
Validate computational predictions or design hypotheses before investing in detailed characterization.
Typical project size: 20-100 variants Goal: Confirm predicted binding behavior
Comparative Studies
Comparative Studies
Compare binding performance across different protein formats, scaffolds, or optimization strategies.
Typical project size: 50-200 variants Goal: Identify most promising approaches
Quality Assessment
Quality Assessment
Screen protein variants to identify those suitable for downstream applications or further optimization.
Typical project size: 10-100 variants Goal: Select well-behaved, binding-competent variants
Complementary Assays
Binding screening often serves as the first step in a comprehensive characterization workflow:
- Affinity Characterization: Detailed kinetic analysis of top performers
- Epitope Binning: Map binding sites of interesting variants
- Thermostability: Assess stability of promising candidates
Data Delivery
Your binding screening results include:
- Binding classification for each variant (Strong/Medium/Weak/Non-binding)
- Relative ranking within each category
- Raw binding curves for visual inspection
- Expression level assessment for each variant
- Summary statistics and quality metrics
- Recommendations for follow-up experiments
Getting Started
Ready to screen your protein variants?
- Prepare your sequences in FASTA or CSV format
- Select your target from our library or provide your own
- Configure your experiment through the Foundry Portal
- Review and submit for rapid turnaround
Binding screening is ideal for new customers or new protein types. Start here to understand how your variants perform on our platform before investing in more detailed assays.
======= description: Protein binding screening is designed to quickly and efficiently evaluate large libraries of protein variants for their ability to bind a target of interest.
This high-throughput workflow enables you to classify candidates as binders or non-binders while also categorizing their binding strength (e.g., weak, medium, or strong). By focusing on rapid and cost-effective assays, our screening service is ideal for early-stage discovery projects where prioritizing candidates is key.
Key Features:
-
Starts at 99$/protein. 21 days turnaround time.
-
Yes/No Binding Classification: Rapidly determine whether each variant binds to the target.
-
Binding Strength Categorization: Classifies binders as weak, medium, or strong (no KD values).
-
High Throughput: Capable of screening thousands of variants in a single campaign.
-
Cost-Effective: Optimized for large-scale projects without compromising data quality.
When to Use:
-
To triage large libraries of protein variants during early discovery phases.
-
To identify promising binders for subsequent detailed characterization.
-
When rapid and broad insights into binding potential are required.
-
If you need precise binding kinetics with KD values, see Affinity Characterization.
How It Works
During a binding screen, protein variants are immobilized or presented in solution, and the target analyte is flowed over the experimental setup. Real-time binding events are monitored using bio-layer interferometry (BLI), and results are analyzed to classify each variant. Single-concentration measurements provide approximate binding strength, allowing you to prioritize strong binders while excluding non-binders or weak candidates
131ed47d083be70c654c1ff1f875b4f3206dfbf8