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Binding Screening

Binding screening is our most popular service, designed for rapid identification and ranking of protein binding interactions. This cost-effective assay is ideal for initial screening of new designs, library exploration, and lead identification campaigns.

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How It Works

Binding screening uses a streamlined protocol with two analyte concentrations to quickly categorize your protein variants into binding strength buckets. This approach provides essential binding information while maintaining high throughput and cost efficiency.

1

Protein Expression

Your protein sequences are expressed using our cell-free system and immobilized on BLI biosensor tips via C-terminal tags.

2

Binding Assessment

Sensors are exposed to two different concentrations of your target protein to assess binding strength and kinetics.

3

Rapid Analysis

Binding responses are analyzed to categorize variants as Strong, Medium, Weak, or Non-binding.

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Binding Classification

Our binding screening categorizes your variants into clear, actionable groups:

KD < 50 nM

High-affinity interactions suitable for therapeutic applications or high-sensitivity assays.

Characteristics:

  • Robust binding signals
  • Clear association and dissociation phases
  • Excellent signal-to-noise ratios
  • High confidence in measurements

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Key Applications

Library Screening

Screen large libraries of protein variants to identify the most promising candidates for further development.

Lead Identification

Rapidly identify binding hits from diverse design approaches or computational predictions.

Comparative Analysis

Compare binding performance across different protein scaffolds, mutations, or design strategies.

Quality Control

Validate protein expression and basic binding functionality before investing in detailed characterization.

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Advantages of Binding Screening

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Speed and Efficiency

  • Rapid turnaround: Results in 2-3 weeks
  • High throughput: Process 100+ variants efficiently
  • Streamlined analysis: Clear categorization without complex fitting

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Cost Effectiveness

  • Lower per-variant cost compared to full characterization
  • Volume discounts for larger screening campaigns
  • Risk reduction by identifying non-binders early

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Decision Support

  • Clear ranking of variant performance
  • Actionable categories for follow-up decisions
  • Statistical confidence in relative rankings

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When to Use Binding Screening

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Complementary Assays

Binding screening often serves as the first step in a comprehensive characterization workflow:

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Data Delivery

Your binding screening results include:

  • Binding classification for each variant (Strong/Medium/Weak/Non-binding)
  • Relative ranking within each category
  • Raw binding curves for visual inspection
  • Expression level assessment for each variant
  • Summary statistics and quality metrics
  • Recommendations for follow-up experiments

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Getting Started

Ready to screen your protein variants?

  1. Prepare your sequences in FASTA or CSV format
  2. Select your target from our library or provide your own
  3. Configure your experiment through the Foundry Portal
  4. Review and submit for rapid turnaround

Binding screening is ideal for new customers or new protein types. Start here to understand how your variants perform on our platform before investing in more detailed assays.

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======= description: Protein binding screening is designed to quickly and efficiently evaluate large libraries of protein variants for their ability to bind a target of interest.

This high-throughput workflow enables you to classify candidates as binders or non-binders while also categorizing their binding strength (e.g., weak, medium, or strong). By focusing on rapid and cost-effective assays, our screening service is ideal for early-stage discovery projects where prioritizing candidates is key.

Key Features:

  • Starts at 99$/protein. 21 days turnaround time.

  • Yes/No Binding Classification: Rapidly determine whether each variant binds to the target.

  • Binding Strength Categorization: Classifies binders as weak, medium, or strong (no KD values).

  • High Throughput: Capable of screening thousands of variants in a single campaign.

  • Cost-Effective: Optimized for large-scale projects without compromising data quality.

When to Use:

  • To triage large libraries of protein variants during early discovery phases.

  • To identify promising binders for subsequent detailed characterization.

  • When rapid and broad insights into binding potential are required.

  • If you need precise binding kinetics with KD values, see Affinity Characterization.

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How It Works

During a binding screen, protein variants are immobilized or presented in solution, and the target analyte is flowed over the experimental setup. Real-time binding events are monitored using bio-layer interferometry (BLI), and results are analyzed to classify each variant. Single-concentration measurements provide approximate binding strength, allowing you to prioritize strong binders while excluding non-binders or weak candidates

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