Project Workflow

The process begins when you submit your project details through the Foundry Portal. Our intuitive configurator allows you to specify:

• Type of experiment: Choose from binding screening, affinity characterization, epitope binning, and more
• Protein variants: Upload sequences or select from our library
• Target molecules: Define the antigens or ligands of interest
• Custom requirements: Set specific concentrations, controls, or replicates

After submission, our team reviews the feasibility of your project, creates a detailed project plan, and communicates any necessary adjustments or recommendations.

During the build phase, we prepare all necessary materials and reagents for your experiments. This includes:

DNA Synthesis and Optimization

• Synthesis of DNA sequences encoding your protein variants
• Codon optimization for efficient expression
• Quality verification of synthesized constructs

Protein Expression

• Production of protein variants using our cell-free expression system
• High-quality, consistent protein yields
• Verification of expression levels and quality

Target Preparation

• Sourcing or production of target molecules
• Access to our pre-curated antigen library or custom target preparation

Buffer and Reagent Preparation

• Tailored to your experimental conditions
• Optimized for stability and performance

This is where we test your proteins and targets using the experimental workflows you’ve selected. For binding experiments, we use bio-layer interferometry (BLI), a label-free technology that provides real-time binding kinetics.

Experiment Execution

• Immobilization or presentation of proteins on biosensor tips
• Introduction of target analytes in solution
• Real-time monitoring of binding interactions

Technical Precision

• Single or multiple technical replicates based on your requirements
• Consistent experimental conditions across all samples

Controls

• Inclusion of negative controls to assess non-specific binding
• Positive controls to validate experimental setup

Quality Assurance

• Continuous monitoring of experimental parameters
• Flagging of unexpected results for review

After data acquisition, we perform rigorous analysis and validation to ensure the accuracy and reliability of your results.

Data Processing

• Fitting of raw sensorgrams to appropriate kinetic models
• Extraction of key parameters (k_on, k_off, K_D values)
• Statistical analysis of replicate data

Validation

• Cross-checking results against controls
• Verification of data consistency across replicates
• Identification of any outliers or anomalies

Comprehensive Reporting

• Detailed report including:
  • Raw data and processed results
  • Kinetic parameters and binding affinities
  • Experimental conditions and notes
  • Interpretation and recommendations

Foundry Portal Access

• View and download raw sensorgrams
• Explore fitted kinetic models
• Filter and categorize results
• Track project status in real-time
• Access archived results anytime