BLI measures real-time protein binding by detecting changes in light interference patterns when molecules bind to a sensor surface.

Method

Label-free optical detection

Data

Real-time kinetics + equilibrium

Sensitivity

Sub-nanomolar detection

Throughput

96-well format available
1

Ligand Immobilization

Target protein attached to biosensor tip
2

Analyte Binding

Test protein flows over sensor surface
3

Signal Detection

Binding causes detectable light interference shift
4

Kinetic Analysis

Association and dissociation rates calculated

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Key Parameters

BLI provides three critical binding parameters: k_on (association), k_off (dissociation), and K_D (equilibrium constant).
  • k_on - Association rate constant
  • k_off - Dissociation rate constant
  • K_D - Equilibrium dissociation constant (k_off/k_on)