Affinity tag purification is a key step in our protein production workflow, enabling rapid, high-purity isolation of target proteins for downstream assays. Our platform uses two primary affinity tag systems optimized for different experimental requirements.

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Supported Affinity Tags

Twin‑Strep‑Tag (Default)

Our default purification tag due to its high affinity and specificity, enabling excellent yields and clean eluates. Highly interoperable with our BLI/SPR measurements.

Other Tags

On demand, we can also work with other tags such as His, Fc, or lab‑specific alternatives. Tag choice depends on your protein and downstream requirements.

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Tag Positioning

C-terminal placement (default): We use C-terminal tags for 90% of proteins because the tag only gets expressed if the entire protein gets expressed. This prevents purification of truncated proteins that could occur with N-terminal tags if protein expression stalls. N-terminal placement (on request): Available through the Foundry portal when needed, such as when the binding site is close to the C-terminus and might be affected by tag placement. All tags include a GS linker for proper spacing and minimal interference with protein function.

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Workflow Integration

Purified proteins from this process are directly used in downstream assays including:
  • Binding assays (BLI, SPR)
  • Structural analysis
  • Functional studies
  • Quality control measurements
Tag selection and positioning can be customized based on your specific protein and experimental requirements through the Foundry portal.