Binding

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Two ways to run binding

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Binding screening (2 concentrations)

• What it is: Fast triage using two target concentrations to sort variants by binding strength.
• When to use: Early discovery and library down‑selection.
• Output: Strong / Medium / Weak / Non‑binder classification with ranked responses.

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Affinity characterization (4+ concentrations)

• What it is: Multi‑concentration series (typically 5–7) with global fitting for precise KD, kon, and koff.
• Why this works: Multiple concentrations across orders of magnitude constrain the fit, separating association (kon) and dissociation (koff). This yields reliable KD across a wider range (≈0.1 nM to 10 µM) than single‑point reads.
• When to use: Lead optimization, benchmarking, and mechanism studies.
• Output: KD, kon, koff with confidence intervals and fit diagnostics.

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Choose the right depth

• Screening: Maximize throughput and minimize cost to find winners.
• Characterization: Quantify kinetics to pick and improve the best.

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Related pages

• Expression
• Thermostability
• Enzyme Activity