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nanoDSF Technology

Our thermostability measurements use nanoDifferential Scanning Fluorimetry (nanoDSF), a label‑free method that tracks intrinsic fluorescence during thermal unfolding.
1

Sample Preparation

Purified proteins are prepared in standard buffer conditions or customer-specified formulations.
2

Temperature Ramping

Samples are subjected to controlled temperature increases while monitoring fluorescence changes.
3

Melting Point Determination

The midpoint of the unfolding transition (Tm) is determined from the fluorescence data.
Curious about the underlying technique? See the wiki: nanoDSF.

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What you get

  • Tm (melting temperature): Primary stability metric
  • Onset temperature and transition width: Context on unfolding behavior
  • Clear ranking: Compare variants for developability decisions

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When to use

  • Selecting robust variants for storage and handling
  • Comparing formulations or mutations for stability impact
  • Pairing with binding or activity to balance function vs stability
Ready to set up a thermostability assay? Visit products.adaptyvbio.com/thermostability.