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For binding experiments, bio-layer interferometry (BLI) is employed to generate real-time data. This stage includes:
  • Experiment Execution: Proteins are immobilized or presented in solution, and target analytes are introduced to monitor interactions.
  • Technical Precision: Assays can include single or multiple replicates to enhance reliability and reproducibility.
  • Controls: Negative and positive controls are included to ensure specificity and accuracy of the results.
Quality Assurance: Every step is carefully monitored to ensure the integrity of the data. Any unexpected results or deviations are flagged for review.