> ## Documentation Index
> Fetch the complete documentation index at: https://docs.adaptyvbio.com/llms.txt
> Use this file to discover all available pages before exploring further.

# Split GFP Quantification

> Split GFP system for direct quantification of protein expression and qualitative yield estimation

Split GFP quantification provides direct quantification of protein expression using a split green fluorescent protein system. We use this technology for expression validation and qualitative yield estimation.

## How It Works

The split GFP system divides the green fluorescent protein into two non-fluorescent fragments. When these fragments are brought together through protein expression, they reconstitute to form a functional, fluorescent GFP. The fluorescence intensity correlates with the amount of expressed protein.

## Applications

<CardGroup cols={2}>
  <Card title="Expression Validation" icon="circle-check">
    Confirm successful protein expression and validate expression.
  </Card>

  <Card title="Yield Estimation" icon="chart-bar">
    Qualitative assessment of protein expression yields across different conditions.
  </Card>
</CardGroup>

## Integration

Split GFP quantification works alongside our other technologies to provide comprehensive protein expression analysis, complementing cell-free expression systems and other analytical methods for complete protein characterization workflows.