> ## Documentation Index
> Fetch the complete documentation index at: https://docs.adaptyvbio.com/llms.txt
> Use this file to discover all available pages before exploring further.

# Thermostability

> Measure thermal stability (Tm) to de-risk development and formulations

## nanoDSF Technology

Our thermostability measurements use **nanoDifferential Scanning Fluorimetry (nanoDSF)**, a label‑free method that tracks intrinsic fluorescence during thermal unfolding.

<Steps>
  <Step title="Sample Preparation">
    Purified proteins are prepared in standard buffer conditions or customer-specified formulations.
  </Step>

  <Step title="Temperature Ramping">
    Samples are subjected to controlled temperature increases while monitoring fluorescence changes.
  </Step>

  <Step title="Melting Point Determination">
    The midpoint of the unfolding transition (Tm) is determined from the fluorescence data.
  </Step>
</Steps>

<Note>
  Curious about the underlying technique? See the wiki: <a href="/wiki/technologies/nanodsf">nanoDSF</a>.
</Note>

## What you get

* **Tm (melting temperature):** Primary stability metric
* **Onset temperature and transition width:** Context on unfolding behavior
* **Clear ranking:** Compare variants for developability decisions

## When to use

* Selecting robust variants for storage and handling
* Comparing formulations or mutations for stability impact
* Pairing with binding or activity to balance function vs stability

## Related pages

* [Expression](/docs/experiment-types/expression)
* [Binding](/docs/experiment-types/binding)
* [Enzyme Activity](/docs/experiment-types/enzyme-activity)

<Callout>
  Ready to set up a thermostability assay? Visit <a href="https://start.adaptyvbio.com/thermostability" target="_blank" rel="noreferrer">start.adaptyvbio.com/thermostability</a>.
</Callout>