> ## Documentation Index
> Fetch the complete documentation index at: https://docs.adaptyvbio.com/llms.txt
> Use this file to discover all available pages before exploring further.

# Expression Scale-Up

> Scale up expression of your best designs and receive purified protein.

Our standard workflow is sequences in, data out: we express just enough protein to run your assays. When you want physical material in hand, we can **scale up expression of selected designs and ship purified protein back to you**.

Scale-up runs on designs we have already expressed, so it follows an [expression](/docs/experiment-types/expression) assay rather than starting from scratch.

## When to use

* You have identified top hits from a screening or characterization campaign and want protein for your own downstream work.
* You need purified material for assays we do not run in-house.

## How it works

<Steps>
  <Step title="Pick your designs">
    Select the designs you want scaled up, typically your best hits from a prior run, so we reuse the DNA and sequences we already have.
  </Step>

  <Step title="We scale and purify">
    We express at a larger volume and purify the protein by affinity tag.
  </Step>

  <Step title="We QC it">
    Every scale-up includes HPLC-SEC and CE-SDS, so you see purity and aggregation state alongside the material.
  </Step>

  <Step title="We ship it to you">
    Purified protein is shipped back in a standard buffer, or a format we agree on for your application.
  </Step>
</Steps>

## Scale options

Three culture volumes are available, small, mid and large. Yield depends heavily on the protein, so each tier carries an expected range rather than a guaranteed amount. Contact us for current volumes, yield ranges and prices.

If you need more material than the large tier delivers, tell us the amount you need and we will scope it.

<Callout>
  Turnaround depends on the protein and the tier. Contact us at <a href="mailto:support@adaptyvbio.com">[support@adaptyvbio.com](mailto:support@adaptyvbio.com)</a>, or through your experiment, to scope a scale-up run.
</Callout>

## Related pages

* [Expression](/docs/experiment-types/expression)
* [Data Deliverables](/docs/experiment-setup/data-deliverables)
* [Supported Protein Formats](/docs/experiment-setup/supported-protein-formats)


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