> ## Documentation Index
> Fetch the complete documentation index at: https://docs.adaptyvbio.com/llms.txt
> Use this file to discover all available pages before exploring further.

# Enzyme Activity

> Quantify catalytic performance with simple, comparable readouts

## What you get

* **Activity call:** Active / low / not detected
* **Relative rate:** Normalized activity per variant for fair comparison
* **Assay conditions:** Substrate, buffer, temperature, and detection method

## Assay formats

* **Endpoint assays:** Single time‑point readouts for high throughput
* **Kinetic assays:** Time‑course measurements for rate estimation

## When to use

* Early triage of designed enzymes to find active scaffolds
* Optimization campaigns to compare mutations and conditions

<CardGroup cols={2}>
  <Card title="Endpoint assay" icon="gauge">
    High‑throughput single time‑point readouts for large libraries.
  </Card>

  <Card title="Kinetic assay" icon="wave-pulse">
    Time‑course data to estimate rates and compare variants.
  </Card>
</CardGroup>

<Callout>
  Ready to set up an enzyme activity assay? Visit <a href="https://start.adaptyvbio.com/enzyme-activity" target="_blank" rel="noreferrer">start.adaptyvbio.com/enzyme-activity</a>.
</Callout>

## Related pages

* [Expression](/docs/experiment-types/expression)
* [Thermostability](/docs/experiment-types/thermostability)